Western blot analysis of extracts of normal 293T cells and 293T transfected with 3CLpro Protein, using SARS-CoV-2 3CLpro antibody (A20198) at 1:5000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 1s.

Immunofluorescence analysis of 293T cells transfected with SARS-CoV-2 3CLpro fusion protein (top left) and untreated 293T cells (top right) use SARS-CoV-2 3CLpro Rabbit pAb (A20198) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Western blot analysis of SARS-CoV-2 3CLpro, using SARS-CoV-2 3CLpro antibody (A20198) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.||Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 1s.