Immobilized Recombinant SARS-COV2 S-ECD-PFS Protein(RP01283LQ) at 1μg/mL (100μL/well) can bind SARS-CoV-2 Spike S2 ECD Rabbit pAb (A20138) with a linear range of 3.12-50ng/mL.

Western blot analysis of extracts of normal 293T cells 293T transfected with Spike S2 ECD Protein, using SARS-CoV-2 Spike S2 ECD antibody (A20138) at 1:5000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Enhanced Kit (RM00021).|Exposure time: 1s.

Immunoprecipitation analysis of 300ug extracts of 293T cells using 3ug SARS-CoV-2 Spike S2 ECD antibody (A20138). Western blot was performed from the immunoprecipitate using SARS-CoV-2 Spike S2 ECD antibody (A20138) at a dilition of 1:5000.

Immunofluorescence analysis of 293T cells transfected with SARS-CoV-2 Spike S2 ECD fusion protein (top left) and untreated 293T cells (top right) use SARS-CoV-2 Spike S2 ECD Rabbit pAb (A20138) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.