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Western blot analysis of extracts from normal (control) and Rad23B knockout (KO) HeLa cells, using Rad23B antibody (A20000) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 90s.
[KO Validated] Rad23B Rabbit pAb
Catalog No：AB-A20000
Manufacturer：ABclonal
Synonyms：RAD23B;HHR23B;HR23B;P58
Applications：WB,IHC,IF
Reactivity：Human,Mouse
Host：Rabbit
Conjugate：No
€65,00 – €485,00
| Product Name: | [KO Validated] Rad23B Rabbit pAb |
| Category: | Polyclonal Antibodies |
| Description: | The protein encoded by this gene is one of two human homologs of Saccharomyces cerevisiae Rad23, a protein involved in the nucleotide excision repair (NER). This protein was found to be a component of the protein complex that specifically complements the NER defect of xeroderma pigmentosum group C (XP-c) cell extracts in vitro. This protein was also shown to interact with, and elevate the nucleotide excision activity of 3-methyladenine-DNA glycosylase (MPG), which suggested a role in DNA damage recognition in base excision repair. This protein contains an N-terminal ubiquitin-like domain, which was reported to interact with 26S proteasome, and thus this protein may be involved in the ubiquitin mediated proteolytic pathway in cells. Alternative splicing results in multiple transcript variants encoding distinct isoforms. |
| Immunogen: | A synthetic peptide of human Rad23B. |
| Gene ID: | 5887 |
| Swiss prot: | P54727 |
| Molecular weight: | 35kDa/43kDa |
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