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Western blot analysis of extracts from normal (control) and B7-H3/CD276 knockout (KO) 293T cells, using B7-H3/CD276 antibody (A19988) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 5s.
[KO Validated] B7-H3/CD276 Rabbit pAb
Catalog No：AB-A19988
Manufacturer：ABclonal
Synonyms：CD276;4Ig-B7-H3;B7-H3;B7H3;B7RP-2
Applications：WB,IF
Reactivity：Human,Mouse
Host：Rabbit
Conjugate：No
€110,00 – €425,00
| Product Name: | [KO Validated] B7-H3/CD276 Rabbit pAb |
| Category: | Polyclonal Antibodies |
| Description: | The protein encoded by this gene belongs to the immunoglobulin superfamily, and thought to participate in the regulation of T-cell-mediated immune response. Studies show that while the transcript of this gene is ubiquitously expressed in normal tissues and solid tumors, the protein is preferentially expressed only in tumor tissues. Additionally, it was observed that the 3′ UTR of this transcript contains a target site for miR29 microRNA, and there is an inverse correlation between the expression of this protein and miR29 levels, suggesting regulation of expression of this gene product by miR29. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. |
| Immunogen: | A synthetic peptide of human B7-H3/CD276. |
| Gene ID: | 80381 |
| Swiss prot: | Q5ZPR3 |
| Molecular weight: | 33kDa/52kDa/57kDa |
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