Western blot analysis of extracts from normal (control) and ARF5 knockout (KO) 293T cells, using ARF5 antibody (A19986) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 5s.
[KO Validated] ARF5 Rabbit pAb
Catalog No:AB-A19986
Manufacturer:ABclonal
Synonyms:ARF5
Applications:WB,IF
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €485,00
Product Name: | [KO Validated] ARF5 Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | This gene is a member of the human ADP-ribosylation factor (ARF) gene family. These genes encode small guanine nucleotide-binding proteins that stimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking and as activators of phospholipase D. The gene products include 6 ARF proteins and 11 ARF-like proteins and constitute 1 family of the RAS superfamily. The ARF proteins are categorized as class I (ARF1, ARF2,and ARF3), class II (ARF4 and ARF5) and class III (ARF6). The members of each class share a common gene organization. |
Immunogen: | Recombinant protein of human ARF5. |
Gene ID: | 381 |
Swiss prot: | P84085 |
Molecular weight: | 20kDa |
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