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Western blot analysis of extracts from normal (control) and PHGDH knockout (KO) HeLa cells, using PHGDH antibody (A19974) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 1s.
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Immunofluorescence analysis of L929 cells using PHGDH antibody (A19974) at dilution of 1:100. Blue: DAPI for nuclear staining.
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Immunofluorescence analysis of H9C2 cells using PHGDH antibody (A19974) at dilution of 1:100. Blue: DAPI for nuclear staining.
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Immunofluorescence analysis of U2OS cells using PHGDH antibody (A19974) at dilution of 1:100. Blue: DAPI for nuclear staining.
[KO Validated] PHGDH Rabbit pAb
Catalog No：AB-A19974
Manufacturer：ABclonal
Synonyms：PHGDH;3-PGDH;3PGDH;HEL-S-113;NLS;NLS1;PDG;PGAD;PGD;PGDH;PHGDHD;SERA
Applications：WB,IF
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
€65,00 – €485,00
| Product Name: | [KO Validated] PHGDH Rabbit pAb |
| Category: | Polyclonal Antibodies |
| Description: | This gene encodes the enzyme which is involved in the early steps of L-serine synthesis in animal cells. L-serine is required for D-serine and other amino acid synthesis. The enzyme requires NAD/NADH as a cofactor and forms homotetramers for activity. Mutations in this gene have been found in a family with congenital microcephaly, psychomotor retardation and other symptoms. Multiple alternatively spliced transcript variants have been found, however the full-length nature of most are not known. |
| Immunogen: | Recombinant protein of human PHGDH. |
| Gene ID: | 26227 |
| Swiss prot: | O43175 |
| Molecular weight: | 56kDa |
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