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Western blot analysis of extracts from normal (control) and VRK1 knockout (KO) 293T cells, using VRK1 antibody (A19938) at 1:500 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 1s.
[KO Validated] VRK1 Rabbit pAb
Catalog No：AB-A19938
Manufacturer：ABclonal
Synonyms：VRK1;PCH1;PCH1A
Applications：WB,IHC
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
€65,00 – €485,00
| Product Name: | [KO Validated] VRK1 Rabbit pAb |
| Category: | Polyclonal Antibodies |
| Description: | This gene encodes a member of the vaccinia-related kinase (VRK) family of serine/threonine protein kinases. This gene is widely expressed in human tissues and has increased expression in actively dividing cells, such as those in testis, thymus, fetal liver, and carcinomas. Its protein localizes to the nucleus and has been shown to promote the stability and nuclear accumulation of a transcriptionally active p53 molecule and, in vitro, to phosphorylate Thr18 of p53 and reduce p53 ubiquitination. This gene, therefore, may regulate cell proliferation. This protein also phosphorylates histone, casein, and the transcription factors ATF2 (activating transcription factor 2) and c-JUN. |
| Immunogen: | Recombinant protein of human VRK1. |
| Gene ID: | 7443 |
| Swiss prot: | Q99986 |
| Molecular weight: | 45kDa |
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