Western blot analysis of extracts from normal (control) and SIPA1 knockout (KO) HeLa cells, using SIPA1 antibody (A19867) at 1:3000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 1s.
[KO Validated] SIPA1 Rabbit pAb
Catalog No:AB-A19867
Manufacturer:ABclonal
Synonyms:SIPA1;SPA1
Applications:WB,IHC
Reactivity:Human,Mouse
Host:Rabbit
Conjugate:No
€65,00 – €485,00
Product Name: | [KO Validated] SIPA1 Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | The product of this gene is a mitogen induced GTPase activating protein (GAP). It exhibits a specific GAP activity for Ras-related regulatory proteins Rap1 and Rap2, but not for Ran or other small GTPases. This protein may also hamper mitogen-induced cell cycle progression when abnormally or prematurely expressed. It is localized to the perinuclear region. Two alternatively spliced variants encoding the same isoform have been characterized to date. |
Immunogen: | Recombinant protein of human SIPA1. |
Gene ID: | 6494 |
Swiss prot: | Q96FS4 |
Molecular weight: | 112kDa |
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