Western blot analysis of extracts of various cell lines, using SUN2 antibody (A19782) at 1:2000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Enhanced Kit (RM00021).|Exposure time: 60s.
SUN2 Rabbit mAb
Catalog No:AB-A19782
Manufacturer:ABclonal
Synonyms:UNC84B
Applications:WB,IHC
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €485,00
Product Name: | SUN2 Rabbit mAb |
Category: | Monoclonal Antibodies |
Description: | SUN1 (MIM 607723) and SUN2 are inner nuclear membrane (INM) proteins that play a major role in nuclear-cytoplasmic connection by formation of a 'bridge' across the nuclear envelope, known as the LINC complex, via interaction with the conserved luminal KASH domain of nesprins (e.g., SYNE1; MIM 608441) located in the outer nuclear membrane (ONM). The LINC complex provides a direct connection between the nuclear lamina and the cytoskeleton, which contributes to nuclear positioning and cellular rigidity (summary by Haque et al., 2010 [PubMed 19933576]).[supplied by OMIM, Nov 2010] |
Immunogen: | A synthesized peptide derived from human SUN2 |
Gene ID: | 25777 |
Swiss prot: | Q9UH99 |
Molecular weight: | 80kDa |
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