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Immunohistochemistry of paraffin-embedded human breast using Ubiquitin antibody (A19686) at dilution of 1:100 (40x lens).
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Immunofluorescence analysis of HeLa cells using Ubiquitin antibody (A19686). Blue: DAPI for nuclear staining.
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Immunofluorescence analysis of NIH-3T3 cells using Ubiquitin antibody (A19686). Blue: DAPI for nuclear staining.
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Western blot analysis of extracts of various cell lines, using Ubiquitin Rabbit mAb (A19686) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 5s.
Ubiquitin Rabbit mAb
Catalog No：AB-A19686
Manufacturer：ABclonal
Synonyms：HEL-S-50;Ubiquitin;UBB
Applications：WB,IHC,IF
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
€65,00 – €485,00
| Product Name: | Ubiquitin Rabbit mAb |
| Category: | Monoclonal Antibodies |
| Description: | This gene encodes ubiquitin, one of the most conserved proteins known. Ubiquitin has a major role in targeting cellular proteins for degradation by the 26S proteosome. It is also involved in the maintenance of chromatin structure, the regulation of gene expression, and the stress response. Ubiquitin is synthesized as a precursor protein consisting of either polyubiquitin chains or a single ubiquitin moiety fused to an unrelated protein. This gene consists of three direct repeats of the ubiquitin coding sequence with no spacer sequence. Consequently, the protein is expressed as a polyubiquitin precursor with a final amino acid after the last repeat. An aberrant form of this protein has been detected in patients with Alzheimer's disease and Down syndrome. Pseudogenes of this gene are located on chromosomes 1, 2, 13, and 17. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2013] |
| Immunogen: | A synthesized peptide derived from human Ubiquitin. |
| Gene ID: | 7314 |
| Swiss prot: | P0CG47 |
| Molecular weight: | 8kDa |
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