Western blot analysis of extracts of various cell lines, using LC3B antibody (A19665) at 1:1000 dilution.293T cells, LC3B knockout (KO) 293T cells, C6 cells and NIH/3T3 cells were treated by Chloroquine (50 μM) at 37℃ for 20 hours.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 5s.
Western blot analysis of extracts of Hela cells, using LC3B pAb (A19665) at 1:1000 dilution.Hela cells were treated by Chloroquine (50 μM) at 37℃ for 20 hours.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit (RM00020).|Exposure time: 5s.
Western blot analysis of extracts from normal (control) and LC3B knockout (KO) 293T cells, using LC3B antibody (A19665) at 1:1000 dilution.293T cells were treated by Chloroquine (50 μM) at 37℃ for 20 hours.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Enhanced Kit (RM00021).|Exposure time: 180s.
Western blot analysis of extracts from normal (control) and LC3B knockout (KO) 293T cells, using LC3B antibody (A19665) at 1:1000 dilution.293T cells were treated by Chloroquine (50 μM) at 37℃ for 20 hours.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 30s.
[KO Validated] LC3B Rabbit mAb
Catalog No:AB-A19665
Manufacturer:ABclonal
Synonyms:LC3B;ATG8F;MAP1LC3B-a;MAP1A/1BLC3;MAP1LC3B;LC3A/LC3B
Applications:WB,IHC,IF
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €485,00
Product Name: | [KO Validated] LC3B Rabbit mAb |
Category: | Monoclonal Antibodies |
Description: | The product of this gene is a subunit of neuronal microtubule-associated MAP1A and MAP1B proteins, which are involved in microtubule assembly and important for neurogenesis. Studies on the rat homolog implicate a role for this gene in autophagy, a process that involves the bulk degradation of cytoplasmic component. |
Immunogen: | A synthetic peptide of human LC3B. |
Gene ID: | 81631 |
Swiss prot: | Q9GZQ8 |
Molecular weight: | 14kDa,16kDa |
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