.jpg)
Western blot analysis of extracts from normal (control) and NF-kB p65/RelA knockout (KO) HeLa cells, using NF-kB p65/RelA antibody (A19653) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 3min.
.jpg)
Western blot analysis of extracts of various cell lines, using NF-kB p65/RelA antibody (A19653) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 3min.
.jpg)
Western blot analysis of extracts of various cell lines, using NF-kB p65/RelA antibody (A19653) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Enhanced Kit (RM00021).|Exposure time: 3min.
.jpg)
Immunohistochemistry of paraffin-embedded rat spleen using NF-kB p65/RelA antibody (A19653) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human lung cancer using NF-kB p65/RelA antibody (A19653) at dilution of 1:100 (40x lens).
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Immunoprecipitation analysis of 200ug extracts of HeLa cells using 3ug NF-kB p65/RelA antibody (A19653). Western blot was performed from the immunoprecipitate using NF-kB p65/RelA antibody (A19653) at a dilition of 1:1000.
[KO Validated] NF-kB p65/RelA Rabbit mAb
Catalog No：AB-A19653
Manufacturer：ABclonal
Synonyms：NFKB3;p65;NF-kB p65;RELA;CMCU
Applications：WB,IHC,IP
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
€65,00 – €1.170,00
| Product Name: | [KO Validated] NF-kB p65/RelA Rabbit mAb |
| Category: | Monoclonal Antibodies |
| Description: | NF-kappa-B is a ubiquitous transcription factor involved in several biological processes. It is held in the cytoplasm in an inactive state by specific inhibitors. Upon degradation of the inhibitor, NF-kappa-B moves to the nucleus and activates transcription of specific genes. NF-kappa-B is composed of NFKB1 or NFKB2 bound to either REL, RELA, or RELB. The most abundant form of NF-kappa-B is NFKB1 complexed with the product of this gene, RELA. Four transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011] |
| Immunogen: | A synthesized peptide derived from human NF-魏B p65. |
| Gene ID: | 5970 |
| Swiss prot: | Q04206 |
| Molecular weight: | 65kDa |
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