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Western blot analysis of extracts of various cell lines, using SP1 antibody (A19649) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 90s.
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Immunohistochemistry of paraffin-embedded rat spleen using SP1 antibody (A19649) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse kidney using SP1 antibody (A19649) at dilution of 1:100 (40x lens).
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Immunofluorescence analysis of C6 cells using SP1 antibody (A19649). Blue: DAPI for nuclear staining.
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Immunofluorescence analysis of NIH-3T3 cells using SP1 antibody (A19649). Blue: DAPI for nuclear staining.
SP1 Rabbit mAb
Catalog No：AB-A19649
Manufacturer：ABclonal
Synonyms：SP1
Applications：WB,IHC,IF
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
€65,00 – €485,00
| Product Name: | SP1 Rabbit mAb |
| Category: | Monoclonal Antibodies |
| Description: | The protein encoded by this gene is a zinc finger transcription factor that binds to GC-rich motifs of many promoters. The encoded protein is involved in many cellular processes, including cell differentiation, cell growth, apoptosis, immune responses, response to DNA damage, and chromatin remodeling. Post-translational modifications such as phosphorylation, acetylation, glycosylation, and proteolytic processing significantly affect the activity of this protein, which can be an activator or a repressor. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2014] |
| Immunogen: | A synthesized peptide derived from human SP1. |
| Gene ID: | 6667 |
| Swiss prot: | P08047 |
| Molecular weight: | 90kDa |
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