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Western blot analysis of extracts of various cell lines, using HDAC2 antibody (A19626) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 3min.
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Immunohistochemistry of paraffin-embedded rat spleen using HDAC2 antibody (A19626) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human oophoroma using HDAC2 antibody (A19626) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse brain using HDAC2 antibody (A19626) at dilution of 1:100 (40x lens).
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Immunofluorescence analysis of C6 cells using HDAC2 antibody (A19626). Blue: DAPI for nuclear staining.
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Immunofluorescence analysis of NIH-3T3 cells using HDAC2 antibody (A19626). Blue: DAPI for nuclear staining.
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Immunoprecipitation analysis of 300ug extracts of HeLa cells using 3ug HDAC2 antibody (A19626). Western blot was performed from the immunoprecipitate using HDAC2 antibody (A19626) at a dilition of 1:1000.
HDAC2 Rabbit mAb
Catalog No：AB-A19626
Manufacturer：ABclonal
Synonyms：HD2;RPD3;YAF1;HDAC2
Applications：WB,IHC,IF,IP
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
€65,00 – €485,00
| Product Name: | HDAC2 Rabbit mAb |
| Category: | Monoclonal Antibodies |
| Description: | This gene product belongs to the histone deacetylase family. Histone deacetylases act via the formation of large multiprotein complexes, and are responsible for the deacetylation of lysine residues at the N-terminal regions of core histones (H2A, H2B, H3 and H4). This protein forms transcriptional repressor complexes by associating with many different proteins, including YY1, a mammalian zinc-finger transcription factor. Thus, it plays an important role in transcriptional regulation, cell cycle progression and developmental events. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2010] |
| Immunogen: | A synthesized peptide derived from human HDAC2. |
| Gene ID: | 3066 |
| Swiss prot: | Q92769 |
| Molecular weight: | 55kDa |
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