.jpg)
Western blot analysis of extracts from normal (control) and p53 knockout (KO) 293T cells, using p53 antibody (A19585) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 10s.
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Immunoprecipitation analysis of 300ug extracts of 293T cells using 3ug p53 antibody (A19585). Western blot was performed from the immunoprecipitate using p53 antibody (A19585) at a dilition of 1:1000.
[KO Validated] p53 Rabbit mAb
Catalog No：AB-A19585
Manufacturer：ABclonal
Synonyms：BCC7;LFS1;P53;TRP53;p53;TP53;BMFS5
Applications：WB,IP
Reactivity：Human
Host：Rabbit
Conjugate：No
€65,00 – €485,00
| Product Name: | [KO Validated] p53 Rabbit mAb |
| Category: | Monoclonal Antibodies |
| Description: | This gene encodes a tumor suppressor protein containing transcriptional activation, DNA binding, and oligomerization domains. The encoded protein responds to diverse cellular stresses to regulate expression of target genes, thereby inducing cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. Mutations in this gene are associated with a variety of human cancers, including hereditary cancers such as Li-Fraumeni syndrome. Alternative splicing of this gene and the use of alternate promoters result in multiple transcript variants and isoforms. Additional isoforms have also been shown to result from the use of alternate translation initiation codons from identical transcript variants (PMIDs: 12032546, 20937277). [provided by RefSeq, Dec 2016] |
| Immunogen: | A synthesized peptide derived from human p53. |
| Gene ID: | 7157 |
| Swiss prot: | P04637 |
| Molecular weight: | 53kDa |
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