Western blot analysis of extracts from normal (control) and Fas knockout (KO) HeLa cells, using Fas antibody (A19582) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 90s.
Western blot analysis of extracts of various cell lines, using Fas antibody (A19582) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 90s.
[KO Validated] Fas Rabbit mAb
Catalog No:AB-A19582
Manufacturer:ABclonal
Synonyms:ALPS1A;APO-1;APT1;CD95;FAS1;FASTM;TNFRSF6;FAS
Applications:WB,IHC
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €485,00
Product Name: | [KO Validated] Fas Rabbit mAb |
Category: | Monoclonal Antibodies |
Description: | The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor contains a death domain. It has been shown to play a central role in the physiological regulation of programmed cell death, and has been implicated in the pathogenesis of various malignancies and diseases of the immune system. The interaction of this receptor with its ligand allows the formation of a death-inducing signaling complex that includes Fas-associated death domain protein (FADD), caspase 8, and caspase 10. The autoproteolytic processing of the caspases in the complex triggers a downstream caspase cascade, and leads to apoptosis. This receptor has been also shown to activate NF-kappaB, MAPK3/ERK1, and MAPK8/JNK, and is found to be involved in transducing the proliferating signals in normal diploid fibroblast and T cells. Several alternatively spliced transcript variants have been described, some of which are candidates for nonsense-mediated mRNA decay (NMD). The isoforms lacking the transmembrane domain may negatively regulate the apoptosis mediated by the full length isoform. [provided by RefSeq, Mar 2011] |
Immunogen: | A synthesized peptide derived from human Fas. |
Gene ID: | 355 |
Swiss prot: | P25445 |
Molecular weight: | 48kDa |
Help & Support
Product
Guarantee and
Expert Support
Related products
Catalog No:AB-A0987
Manufacturer:ABclonal
Synonyms:DJ-1;DJ1;GATD2;HEL-S-67p;PARK7
Applications:WB,IF
Reactivity:Human,Mouse
Host:Rabbit
Conjugate:No
€65,00 – €485,00
Catalog No:AB-A0533
Manufacturer:ABclonal
Synonyms:HK, HK1-ta, HK1-tb, HK1-tc, HKD, HKI, HMSNR, HXK1, hexokinase
Applications:WB,IHC,IF,IP
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €605,00
Catalog No:AB-A0432
Manufacturer:ABclonal
Synonyms:CD325;CDHN;CDw325;NCAD;N Cadherin;CDH2
Applications:WB,IHC,IF
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €485,00
Catalog No:AB-A0228
Manufacturer:ABclonal
Synonyms:ERK-1;ERK1;ERT2;HS44KDAP;HUMKER1A;P44ERK1;P44MAPK;PRKM3;p44-ERK1;p44-MAPK;MAPK3
Applications:WB,IHC,IF
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €485,00
Catalog No:AB-A0207
Manufacturer:ABclonal
Synonyms:BCL2L4;BAX
Applications:WB,IHC,IF,IP
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €485,00
Catalog No:AB-A0003
Manufacturer:ABclonal
Synonyms:OSRC;PPP1R130;RB;p105-Rb;pRb;pp110;RB1;Rb
Applications:WB,IF
Reactivity:Human
Host:Rabbit
Conjugate:No
€65,00 – €485,00