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Western blot analysis of extracts of various cell lines, using Histone H2B antibody (A1958) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 10s.
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Western blot analysis of extracts from normal (control) and Histone H2B Rabbit pAb knockout (KO) 293T cells, using Histone H2B Rabbit pAb antibody (A1958) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 2s.
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Immunohistochemistry of paraffin-embedded human esophagus using Histone H2B antibody (A1958) at dilution of 1:100 (40x lens).
Western blot analysis of extracts from normal (control) and Histone H2B Rabbit pAb knockout (KO) 293T cells, using Histone H2B Rabbit pAb antibody (A1958) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 2s.
Immunohistochemistry of paraffin-embedded human esophagus using Histone H2B antibody (A1958) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded rat kidney using Histone H2B antibody (A1958) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse liver using Histone H2B antibody (A1958) at dilution of 1:100 (40x lens).
[KO Validated] Histone H2B Rabbit pAb
Catalog No：AB-A1958
Manufacturer：ABclonal
Synonyms：GL105;H2B;H2B.1;H2BFQ;H2BGL105;H2BQ;Histone H2B;HIST2H2BE
Applications：WB,IHC
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
€65,00 – €370,00
| Product Name: | [KO Validated] Histone H2B Rabbit pAb |
| Category: | Polyclonal Antibodies |
| Description: | Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a replication-dependent histone that is a member of the histone H2B family, and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif. The protein has antibacterial and antifungal antimicrobial activity. |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-126 of human HIST2H2BE (NP_003519.1). |
| Gene ID: | 8349 |
| Swiss prot: | Q16778 |
| Molecular weight: | 13kDa |
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