Western blot analysis of extracts of various cell lines, using Insulin antibody (A19066) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 3min.
Insulin Rabbit mAb
Catalog No:AB-A19066
Manufacturer:ABclonal
Synonyms:IDDM;IDDM1;IDDM2;ILPR;IRDN;MODY10;INS;Insulin;insulin
Applications:WB,IHC,IF
Reactivity:Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €485,00
Product Name: | Insulin Rabbit mAb |
Category: | Monoclonal Antibodies |
Description: | After removal of the precursor signal peptide, proinsulin is post-translationally cleaved into three peptides: the B chain and A chain peptides, which are covalently linked via two disulfide bonds to form insulin, and C-peptide. Binding of insulin to the insulin receptor (INSR) stimulates glucose uptake. A multitude of mutant alleles with phenotypic effects have been identified. There is a read-through gene, INS-IGF2, which overlaps with this gene at the 5' region and with the IGF2 gene at the 3' region. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2010] |
Immunogen: | A synthesized peptide derived from human Insulin |
Gene ID: | 3630 |
Swiss prot: | P01308 |
Molecular weight: | kDa |
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