Western blot analysis of extracts of various cell lines, using Fascin/Fascin/FSCN1 antibody (A1904) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 30s.
Western blot analysis of extracts of various cell lines, using Fascin/Fascin/FSCN1 antibody (A1904) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 30s.
Western blot analysis of extracts from normal (control) and Fascin/Fascin/FSCN1 knockout (KO) HeLa cells, using Fascin/Fascin/FSCN1 antibody (A1904) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 5s.
[KO Validated] Fascin/FSCN1 Rabbit pAb
Catalog No:AB-A1904
Manufacturer:ABclonal
Synonyms:FSCN1;FAN1;HSN;SNL;p55;fascin
Applications:WB,IHC,IF
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €2.340,00
Product Name: | [KO Validated] Fascin/FSCN1 Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | This gene encodes a member of the fascin family of actin-binding proteins. Fascin proteins organize F-actin into parallel bundles, and are required for the formation of actin-based cellular protrusions. The encoded protein plays a critical role in cell migration, motility, adhesion and cellular interactions. Expression of this gene is known to be regulated by several microRNAs, and overexpression of this gene may play a role in the metastasis of multiple types of cancer by increasing cell motility. Expression of this gene is also a marker for Reed-Sternberg cells in Hodgkin’s lymphoma. A pseudogene of this gene is located on the long arm of chromosome 15. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 394-493 of human Fascin/Fascin/FSCN1 (NP_003079.1). |
Gene ID: | 6624 |
Swiss prot: | Q16658 |
Molecular weight: | 54kDa |
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