Western blot analysis of extracts from normal (control) and CD44 knockout (KO) HeLa cells, using CD44 antibody (A19020) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 3min.
Western blot analysis of extracts of U-251MG cells, using CD44 antibody (A19020) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 3min.
[KO Validated] CD44 Rabbit mAb
Catalog No:AB-A19020
Manufacturer:ABclonal
Synonyms:CDW44;CSPG8;ECMR-III;HCELL;HUTCH-I;IN;LHR;MC56;MDU2;MDU3;MIC4;Pgp1;CD44;CD44
Applications:WB,IHC
Reactivity:Human
Host:Rabbit
Conjugate:No
€65,00 – €485,00
Product Name: | [KO Validated] CD44 Rabbit mAb |
Category: | Monoclonal Antibodies |
Description: | The protein encoded by this gene is a cell-surface glycoprotein involved in cell-cell interactions, cell adhesion and migration. It is a receptor for hyaluronic acid (HA) and can also interact with other ligands, such as osteopontin, collagens, and matrix metalloproteinases (MMPs). This protein participates in a wide variety of cellular functions including lymphocyte activation, recirculation and homing, hematopoiesis, and tumor metastasis. Transcripts for this gene undergo complex alternative splicing that results in many functionally distinct isoforms, however, the full length nature of some of these variants has not been determined. Alternative splicing is the basis for the structural and functional diversity of this protein, and may be related to tumor metastasis. [provided by RefSeq, Jul 2008] |
Immunogen: | A synthesized peptide derived from human CD44 |
Gene ID: | 960 |
Swiss prot: | P16070 |
Molecular weight: | 82kDa |
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