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Western blot analysis of extracts of various cell lines, using PDHA1 antibody (A1895) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.
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Immunohistochemistry of paraffin-embedded human colon using PDHA1 Rabbit pAb (A1895) at dilution of 1:100 (40x lens).
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Immunofluorescence analysis of NIH-3T3 cells using PDHA1 antibody (A1895) at dilution of 1:100. Blue: DAPI for nuclear staining.
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Immunofluorescence analysis of U-2 OS cells using PDHA1 antibody (A1895) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunohistochemistry of paraffin-embedded human colon using PDHA1 Rabbit pAb (A1895) at dilution of 1:100 (40x lens).
PDHA1 Rabbit pAb
Catalog No：AB-A1895
Manufacturer：ABclonal
Synonyms：PDHA1;PDHA;PDHAD;PDHCE1A;PHE1A
Applications：WB,IHC,IF,IP
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
€65,00 – €370,00
| Product Name: | PDHA1 Rabbit pAb |
| Category: | Polyclonal Antibodies |
| Description: | The pyruvate dehydrogenase (PDH) complex is a nuclear-encoded mitochondrial multienzyme complex that catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2), and provides the primary link between glycolysis and the tricarboxylic acid (TCA) cycle. The PDH complex is composed of multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3). The E1 enzyme is a heterotetramer of two alpha and two beta subunits. This gene encodes the E1 alpha 1 subunit containing the E1 active site, and plays a key role in the function of the PDH complex. Mutations in this gene are associated with pyruvate dehydrogenase E1-alpha deficiency and X-linked Leigh syndrome. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 30-390 of human PDHA1 (NP_000275.1). |
| Gene ID: | 5160 |
| Swiss prot: | P08559 |
| Molecular weight: | 40kDa/43kDa/44kDa/47kDa |
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