Western blot analysis of extracts of various cell lines, using Rad21 antibody (A18850) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 1s.
Rad21 Rabbit pAb
Catalog No:AB-A18850
Manufacturer:ABclonal
Synonyms:CDLS4, HR21, HRAD21, MCD1, NXP1, SCC1, hHR21
Applications:WB,IF
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | Rad21 Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | The protein encoded by this gene is highly similar to the gene product of Schizosaccharomyces pombe rad21, a gene involved in the repair of DNA double-strand breaks, as well as in chromatid cohesion during mitosis. This protein is a nuclear phospho-protein, which becomes hyperphosphorylated in cell cycle M phase. The highly regulated association of this protein with mitotic chromatin specifically at the centromere region suggests its role in sister chromatid cohesion in mitotic cells. [provided by RefSeq, Jul 2008] |
Immunogen: | Recombinant protein of human Rad21 . |
Gene ID: | 5885 |
Swiss prot: | O60216 |
Molecular weight: |
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