Western blot analysis of extracts of various cell lines, using NOTCH3 antibody (A0929) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Enhanced Kit (RM00021).|Exposure time: 15s.
NOTCH3 Rabbit pAb
Catalog No:AB-A0929
Manufacturer:ABclonal
Synonyms:NOTCH3;CADASIL;CADASIL1;CASIL;IMF2;LMNS;notch 3
Applications:WB,IF
Reactivity:Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | NOTCH3 Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | This gene encodes the third discovered human homologue of the Drosophilia melanogaster type I membrane protein notch. In Drosophilia, notch interaction with its cell-bound ligands (delta, serrate) establishes an intercellular signalling pathway that plays a key role in neural development. Homologues of the notch-ligands have also been identified in human, but precise interactions between these ligands and the human notch homologues remains to be determined. Mutations in NOTCH3 have been identified as the underlying cause of cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy (CADASIL). |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 751-850 of human NOTCH3 (NP_000426.2). |
Gene ID: | 4854 |
Swiss prot: | Q9UM47 |
Molecular weight: | 243kDa |
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