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Western blot analysis of extracts of various cell lines, using TRIM5 antibody (A1872) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 60s.
TRIM5 Rabbit pAb
Catalog No：AB-A1872
Manufacturer：ABclonal
Synonyms：TRIM5;RNF88;TRIM5alpha
Applications：WB
Reactivity：Human,Mouse
Host：Rabbit
Conjugate：No
€65,00 – €370,00
| Product Name: | TRIM5 Rabbit pAb |
| Category: | Polyclonal Antibodies |
| Description: | The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein forms homo-oligomers via the coilel-coil region and localizes to cytoplasmic bodies. It appears to function as a E3 ubiquitin-ligase and ubiqutinates itself to regulate its subcellular localization. It may play a role in retroviral restriction. Multiple alternatively spliced transcript variants encoding different isoforms have been described for this gene. |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 100-200 of human TRIM5 (NP_149083.2). |
| Gene ID: | 85363 |
| Swiss prot: | Q9C035 |
| Molecular weight: | 29kDa/31kDa/37kDa/40kDa/46kDa/56kDa |
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