Western blot analysis of extracts of various cell lines, using SUPT16H (SPT16) antibody (A18696) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 1s.
Western blot analysis of extracts of Mouse brain, using SUPT16H (SPT16) antibody (A18696) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 30s.
SUPT16H (SPT16) Rabbit pAb
Catalog No:AB-A18696
Manufacturer:ABclonal
Synonyms:CDC68; SPT16; FACTP140; SPT16/CDC68
Applications:WB,IF
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | SUPT16H (SPT16) Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | Transcription of protein-coding genes can be reconstituted on naked DNA with only the general transcription factors and RNA polymerase II. However, this minimal system cannot transcribe DNA packaged into chromatin, indicating that accessory factors may facilitate access to DNA. One such factor, FACT (facilitates chromatin transcription), interacts specifically with histones H2A/H2B to effect nucleosome disassembly and transcription elongation. FACT is composed of an 80 kDa subunit and a 140 kDa subunit; this gene encodes the 140 kDa subunit. |
Immunogen: | Recombinant protein of human SUPT16H (SPT16). |
Gene ID: | 11198 |
Swiss prot: | Q9Y5B9 |
Molecular weight: | 119kDa |
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