Western blot analysis of extracts of various cell lines, using Calpain 2 antibody (A1861) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 30s.
Western blot analysis of extracts from normal (control) and Calpain 2 knockout (KO) 293T cells, using Calpain 2 antibody (A1861) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 1s.
[KO Validated] Calpain 2 Rabbit pAb
Catalog No:AB-A1861
Manufacturer:ABclonal
Synonyms:CAPN2;CANP2;CANPL2;CANPml;mCANP;calpain 2
Applications:WB,IHC,IF
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €485,00
Product Name: | [KO Validated] Calpain 2 Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | The calpains, calcium-activated neutral proteases, are nonlysosomal, intracellular cysteine proteases. The mammalian calpains include ubiquitous, stomach-specific, and muscle-specific proteins. The ubiquitous enzymes consist of heterodimers with distinct large, catalytic subunits associated with a common small, regulatory subunit. This gene encodes the large subunit of the ubiquitous enzyme, calpain 2. Multiple heterogeneous transcriptional start sites in the 5′ UTR have been reported. Two transcript variants encoding different isoforms have been found for this gene. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 350-680 of human Calpain 2 (NP_001739.2). |
Gene ID: | 824 |
Swiss prot: | P17655 |
Molecular weight: | 71kDa/79kDa |
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