Western blot analysis of extracts of various cell lines, using Ikaros antibody (A1850) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 3min.
Ikaros Rabbit pAb
Catalog No:AB-A1850
Manufacturer:ABclonal
Synonyms:IKZF1;CVID13;Hs.54452;IK1;IKAROS;LYF1;LyF-1;PPP1R92;PRO0758;ZNFN1A1
Applications:WB,IF
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | Ikaros Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | This gene encodes a transcription factor that belongs to the family of zinc-finger DNA-binding proteins associated with chromatin remodeling. The expression of this protein is restricted to the fetal and adult hemo-lymphopoietic system, and it functions as a regulator of lymphocyte differentiation. Several alternatively spliced transcript variants encoding different isoforms have been described for this gene. Most isoforms share a common C-terminal domain, which contains two zinc finger motifs that are required for hetero- or homo-dimerization, and for interactions with other proteins. The isoforms, however, differ in the number of N-terminal zinc finger motifs that bind DNA and in nuclear localization signal presence, resulting in members with and without DNA-binding properties. Only a few isoforms contain the requisite three or more N-terminal zinc motifs that confer high affinity binding to a specific core DNA sequence element in the promoters of target genes. The non-DNA-binding isoforms are largely found in the cytoplasm, and are thought to function as dominant-negative factors. Overexpression of some dominant-negative isoforms have been associated with B-cell malignancies, such as acute lymphoblastic leukemia (ALL). |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human Ikaros (NP_001207694.1). |
Gene ID: | 10320 |
Swiss prot: | Q13422 |
Molecular weight: | 24-31kDa/41-57kDa |
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