Western blot analysis of extracts of various cell lines, using Fibrillarin/U3 RNP Rabbit mAb (A0850) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 10s.
Western blot analysis of extracts of various cell lines, using Fibrillarin/U3 RNP Rabbit mAb (A0850) at 1:5000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 5s.
Fibrillarin/U3 RNP Rabbit mAb
Catalog No:AB-A0850
Manufacturer:ABclonal
Synonyms:FIB, FLRN, RNU3IP1
Applications:WB,IF
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €485,00
Product Name: | Fibrillarin/U3 RNP Rabbit mAb |
Category: | Monoclonal Antibodies |
Description: | This gene product is a component of a nucleolar small nuclear ribonucleoprotein (snRNP) particle thought to participate in the first step in processing preribosomal RNA. It is associated with the U3, U8, and U13 small nuclear RNAs and is located in the dense fibrillar component (DFC) of the nucleolus. The encoded protein contains an N-terminal repetitive domain that is rich in glycine and arginine residues, like fibrillarins in other species. Its central region resembles an RNA-binding domain and contains an RNP consensus sequence. Antisera from approximately 8% of humans with the autoimmune disease scleroderma recognize fibrillarin. [provided by RefSeq, Jul 2008] |
Immunogen: | A synthesized peptide derived from human Fibrillarin/U3 RNP |
Gene ID: | 2091 |
Swiss prot: | P22087 |
Molecular weight: | 33kDa |
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