Western blot analysis of extracts of various cell lines, using IRF3 antibody (A0816) at 1:3000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 1s.
Western blot analysis of extracts from normal (control) and IRF3 Rabbit pAb knockout (KO) HeLa cells, using IRF3 Rabbit pAb antibody (A0816) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 5s.
[KO Validated] IRF3 Rabbit pAb
Catalog No:AB-A0816
Manufacturer:ABclonal
Synonyms:IIAE7;IRF3
Applications:WB,IHC,IF
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | [KO Validated] IRF3 Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | This gene encodes a member of the interferon regulatory transcription factor (IRF) family. The encoded protein is found in an inactive cytoplasmic form that upon serine/threonine phosphorylation forms a complex with CREBBP. This complex translocates to the nucleus and activates the transcription of interferons alpha and beta, as well as other interferon-induced genes. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 100-200 of human IRF3 (NP_001184051.1). |
Gene ID: | 3661 |
Swiss prot: | Q14653 |
Molecular weight: | 12kDa/16kDa/33kDa/47kDa/49kDa |
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