Western blot analysis of extracts of various cell lines, using JAK1 antibody (A18323) at 1:500 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 3min.
Western blot analysis of extracts from normal (control) and JAK1 knockout (KO) HeLa cells, using JAK1 antibody (A18323) at 1:500 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Enhanced Kit (RM00021).|Exposure time: 30s.
[KO Validated] JAK1 Rabbit pAb
Catalog No:AB-A18323
Manufacturer:ABclonal
Synonyms:JAK1;JAK1A;JAK1B;JTK3
Applications:WB,IF
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | [KO Validated] JAK1 Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | This gene encodes a membrane protein that is a member of a class of protein-tyrosine kinases (PTK) characterized by the presence of a second phosphotransferase-related domain immediately N-terminal to the PTK domain. The encoded kinase phosphorylates STAT proteins (signal transducers and activators of transcription) and plays a key role in interferon-alpha/beta and interferon-gamma signal transduction. Alternative splicing results in multiple transcript variants. |
Immunogen: | Recombinant protein of human JAK1. |
Gene ID: | 3716 |
Swiss prot: | P23458 |
Molecular weight: | 133kDa |
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