Western blot analysis of extracts of HeLa cells, using Cdc6 antibody (A18249) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 1s.
Cdc6 Rabbit pAb
Catalog No:AB-A18249
Manufacturer:ABclonal
Synonyms:CDC18L;HsCDC6;MGORS5;HsCDC18;CDC6
Applications:WB
Reactivity:Human
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | Cdc6 Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | The protein encoded by this gene is highly similar to Saccharomyces cerevisiae Cdc6, a protein essential for the initiation of DNA replication. This protein functions as a regulator at the early steps of DNA replication. It localizes in cell nucleus during cell cyle G1, but translocates to the cytoplasm at the start of S phase. The subcellular translocation of this protein during cell cyle is regulated through its phosphorylation by Cdks. Transcription of this protein was reported to be regulated in response to mitogenic signals through transcriptional control mechanism involving E2F proteins. |
Immunogen: | Recombinant protein of human Cdc6. |
Gene ID: | 990 |
Swiss prot: | Q99741 |
Molecular weight: | 62kDa |
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