Western blot analysis of extracts of various cell lines, using NBS1/NBS1/NBN antibody (A0783) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 90s.
Western blot analysis of extracts from normal (control) and NBS1/NBS1/NBN knockout (KO) 293T cells, using NBS1/NBS1/NBN antibody (A0783) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 90s.
[KO Validated] NBS1/NBN Rabbit pAb
Catalog No:AB-A0783
Manufacturer:ABclonal
Synonyms:NBN;AT-V1;AT-V2;ATV;NBS;NBS1;P95;nibrin
Applications:WB,IHC
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €485,00
Product Name: | [KO Validated] NBS1/NBN Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | Mutations in this gene are associated with Nijmegen breakage syndrome, an autosomal recessive chromosomal instability syndrome characterized by microcephaly, growth retardation, immunodeficiency, and cancer predisposition. The encoded protein is a member of the MRE11/RAD50 double-strand break repair complex which consists of 5 proteins. This gene product is thought to be involved in DNA double-strand break repair and DNA damage-induced checkpoint activation. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 435-754 of human NBS1/NBS1/NBN (NP_002476.2). |
Gene ID: | 4683 |
Swiss prot: | O60934 |
Molecular weight: | 84kDa |
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