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Western blot analysis of extracts of various cell lines, using BTRC antibody (A18232) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 30s.
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Immunohistochemistry of paraffin-embedded Mouse heart using BTRC Rabbit pAb (A18232) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded Rat heart using BTRC Rabbit pAb (A18232) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded Human colon using BTRC Rabbit pAb (A18232) at dilution of 1:100 (40x lens).
BTRC Rabbit pAb
Catalog No：AB-A18232
Manufacturer：ABclonal
Synonyms：BTRC;BETA-TRCP;FBW1A;FBXW1;FBXW1A;FWD1;bTrCP;bTrCP1;betaTrCP;Beta-TRCP
Applications：WB,IHC,IF
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
€65,00 – €370,00
| Product Name: | BTRC Rabbit pAb |
| Category: | Polyclonal Antibodies |
| Description: | This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbws class; in addition to an F-box, this protein contains multiple WD-40 repeats. The encoded protein mediates degradation of CD4 via its interaction with HIV-1 Vpu. It has also been shown to ubiquitinate phosphorylated NFKBIA (nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha), targeting it for degradation and thus activating nuclear factor kappa-B. Alternatively spliced transcript variants have been described. A related pseudogene exists in chromosome 6. |
| Immunogen: | Recombinant protein of human BTRC. |
| Gene ID: | 8945 |
| Swiss prot: | Q9Y297 |
| Molecular weight: | 68kDa |
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