Western blot analysis of extracts from normal (control) and PAK4 knockout (KO) 293T cells, using PAK4 antibody (A18059) at 1:500 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 3min.
[KO Validated] PAK4 Rabbit pAb
Catalog No:AB-A18059
Manufacturer:ABclonal
Synonyms:PAK4
Applications:WB
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €485,00
Product Name: | [KO Validated] PAK4 Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | PAK proteins, a family of serine/threonine p21-activating kinases, include PAK1, PAK2, PAK3 and PAK4. PAK proteins are critical effectors that link Rho GTPases to cytoskeleton reorganization and nuclear signaling. They serve as targets for the small GTP binding proteins Cdc42 and Rac and have been implicated in a wide range of biological activities. PAK4 interacts specifically with the GTP-bound form of Cdc42Hs and weakly activates the JNK family of MAP kinases. PAK4 is a mediator of filopodia formation and may play a role in the reorganization of the actin cytoskeleton. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. |
Immunogen: | Recombinant protein of human PAK4 |
Gene ID: | 10298 |
Swiss prot: | O96013 |
Molecular weight: | 47kDa/48kDa/54kDa/64kDa |
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