Western blot analysis of extracts from normal (control) and Prion Protein knockout (KO) HeLa cells, using Prion Protein antibody (A18058) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 180s.
[KO Validated] Prion Protein Rabbit pAb
Catalog No:AB-A18058
Manufacturer:ABclonal
Synonyms:PRNP;ASCR;AltPrP;CD230;CJD;GSS;KURU;PRIP;PrP;PrP27-30;PrP33-35C;PrPc;p27-30
Applications:WB,IHC
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €485,00
Product Name: | [KO Validated] Prion Protein Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | The protein encoded by this gene is a membrane glycosylphosphatidylinositol-anchored glycoprotein that tends to aggregate into rod-like structures. The encoded protein contains a highly unstable region of five tandem octapeptide repeats. This gene is found on chromosome 20, approximately 20 kbp upstream of a gene which encodes a biochemically and structurally similar protein to the one encoded by this gene. Mutations in the repeat region as well as elsewhere in this gene have been associated with Creutzfeldt-Jakob disease, fatal familial insomnia, Gerstmann-Straussler disease, Huntington disease-like 1, and kuru. An overlapping open reading frame has been found for this gene that encodes a smaller, structurally unrelated protein, AltPrp. Alternative splicing results in multiple transcript variants. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 23-230 of human Prion Protein (NP_001073592.1). |
Gene ID: | 5621 |
Swiss prot: | P04156 |
Molecular weight: | 8kDa/26kDa/27kDa |
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