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Western blot analysis of extracts from normal (control) and HERC4 knockout (KO) HeLa cells, using HERC4 antibody (A18049) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 1min.
[KO Validated] HERC4 Rabbit pAb
Catalog No：AB-A18049
Manufacturer：ABclonal
Synonyms：HERC4
Applications：WB
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
€65,00 – €485,00
| Product Name: | [KO Validated] HERC4 Rabbit pAb |
| Category: | Polyclonal Antibodies |
| Description: | HERC4 belongs to the HERC family of ubiquitin ligases, all of which contain a HECT domain and at least 1 RCC1 (MIM 179710)-like domain (RLD). The 350-amino acid HECT domain is predicted to catalyze the formation of a thioester with ubiquitin before transferring it to a substrate, and the RLD is predicted to act as a guanine nucleotide exchange factor for small G proteins (Hochrainer et al., 2005 [PubMed 15676274]).[supplied by OMIM, Mar 2008] |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 400-700 of human HERC4 (NP_056416.2). |
| Gene ID: | 26091 |
| Swiss prot: | Q5GLZ8 |
| Molecular weight: | 12kDa/13kDa/106kDa/109kDa/117kDa/118kDa |
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