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Western blot analysis of extracts from normal (control) and MAP3K1 knockout (KO) HeLa cells, using MAP3K1 antibody (A18041) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 1min.
[KO Validated] MAP3K1 Rabbit pAb
Catalog No：AB-A18041
Manufacturer：ABclonal
Synonyms：MAP3K1;MAPKKK1;MEKK;MEKK 1;MEKK1;SRXY6
Applications：WB
Reactivity：Human,Mouse
Host：Rabbit
Conjugate：No
€65,00 – €485,00
| Product Name: | [KO Validated] MAP3K1 Rabbit pAb |
| Category: | Polyclonal Antibodies |
| Description: | The protein encoded by this gene is a serine/threonine kinase and is part of some signal transduction cascades, including the ERK and JNK kinase pathways as well as the NF-kappa-B pathway. The encoded protein is activated by autophosphorylation and requires magnesium as a cofactor in phosphorylating other proteins. This protein has E3 ligase activity conferred by a plant homeodomain (PHD) in its N-terminus and phospho-kinase activity conferred by a kinase domain in its C-terminus. |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 1400 to the C-terminus of human MAP3K1 (NP_005912.1). |
| Gene ID: | 4214 |
| Swiss prot: | Q13233 |
| Molecular weight: | 164kDa |
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