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Western blot analysis of extracts from normal (control) and TRIM21 knockout (KO) A-549 cells, using TRIM21 antibody (A18027) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 1s.
[KO Validated] TRIM21 Rabbit pAb
Catalog No：AB-A18027
Manufacturer：ABclonal
Synonyms：TRIM21;RNF81;RO52;Ro/SSA;SSA;SSA1
Applications：WB,IHC,IF
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
€65,00 – €485,00
| Product Name: | [KO Validated] TRIM21 Rabbit pAb |
| Category: | Polyclonal Antibodies |
| Description: | This gene encodes a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The encoded protein is part of the RoSSA ribonucleoprotein, which includes a single polypeptide and one of four small RNA molecules. The RoSSA particle localizes to both the cytoplasm and the nucleus. RoSSA interacts with autoantigens in patients with Sjogren syndrome and systemic lupus erythematosus. Alternatively spliced transcript variants for this gene have been described but the full-length nature of only one has been determined. |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 80-330 of human TRIM21 (NP_003132.2). |
| Gene ID: | 6737 |
| Swiss prot: | P19474 |
| Molecular weight: | 45kDa/54kDa |
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