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Western blot analysis of extracts from normal (control) and Sonic Hedgehog (Sonic Hedgehog (Shh)) knockout (KO) HeLa cells, using Sonic Hedgehog (Sonic Hedgehog (Shh)) antibody (A18020) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 10s.
[KO Validated] Sonic Hedgehog (Shh) Rabbit pAb
Catalog No：AB-A18020
Manufacturer：ABclonal
Synonyms：SHH;HHG1;HLP3;HPE3;MCOPCB5;SMMCI;TPT;TPTPS
Applications：WB,IHC
Reactivity：Human
Host：Rabbit
Conjugate：No
€65,00 – €485,00
| Product Name: | [KO Validated] Sonic Hedgehog (Shh) Rabbit pAb |
| Category: | Polyclonal Antibodies |
| Description: | This gene encodes a protein that is instrumental in patterning the early embryo. It has been implicated as the key inductive signal in patterning of the ventral neural tube, the anterior-posterior limb axis, and the ventral somites. Of three human proteins showing sequence and functional similarity to the sonic hedgehog protein of Drosophila, this protein is the most similar. The protein is made as a precursor that is autocatalytically cleaved; the N-terminal portion is soluble and contains the signalling activity while the C-terminal portion is involved in precursor processing. More importantly, the C-terminal product covalently attaches a cholesterol moiety to the N-terminal product, restricting the N-terminal product to the cell surface and preventing it from freely diffusing throughout the developing embryo. Defects in this protein or in its signalling pathway are a cause of holoprosencephaly (HPE). It is also thought that mutations in this gene or in its signalling pathway may be responsible for VACTERL syndrome. Additionally, mutations in a long range enhancer located approximately 1 megabase upstream of this gene disrupt limb patterning and can result in preaxial polydactyly. |
| Immunogen: | A synthetic peptide of human Sonic Hedgehog (Sonic Hedgehog (Shh)) |
| Gene ID: | 6469 |
| Swiss prot: | Q15465 |
| Molecular weight: | 49kDa |
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