Western blot analysis of extracts from normal (control) and NME1/NM23A knockout (KO) 293T cells, using NME1/NM23A antibody (A17999) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 5s.
[KO Validated] NME1/NM23A Rabbit pAb
Catalog No:AB-A17999
Manufacturer:ABclonal
Synonyms:NME1;AWD;GAAD;NB;NBS;NDKA;NDPK-A;NDPKA;NM23;NM23-H1
Applications:WB,IHC,IF
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €485,00
Product Name: | [KO Validated] NME1/NM23A Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | This gene (NME1) was identified because of its reduced mRNA transcript levels in highly metastatic cells. Nucleoside diphosphate kinase (NDK) exists as a hexamer composed of ‘A’ (encoded by this gene) and ‘B’ (encoded by NME2) isoforms. Mutations in this gene have been identified in aggressive neuroblastomas. Two transcript variants encoding different isoforms have been found for this gene. Co-transcription of this gene and the neighboring downstream gene (NME2) generates naturally-occurring transcripts (NME1-NME2), which encodes a fusion protein comprised of sequence sharing identity with each individual gene product. |
Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 90 to the C-terminus of human NME1/NM23A (NP_000260.1). |
Gene ID: | 4830 |
Swiss prot: | P15531 |
Molecular weight: | 17kDa/19kDa/30kDa |
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