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Western blot analysis of extracts of Rat liver, using ZO-1 antibody (A0659) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 30s.
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Western blot analysis of extracts of various cell lines, using ZO-1 antibody (A0659) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Enhanced Kit (RM00021).|Exposure time: 15s.
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Immunohistochemistry of paraffin-embedded Mouse stomach using ZO-1 antibody (A0659) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded Rat stomach using ZO-1 antibody (A0659) at dilution of 1:100 (40x lens).
ZO-1 Rabbit pAb
Catalog No：AB-A0659
Manufacturer：ABclonal
Synonyms：TJP1;ZO-1
Applications：WB,IHC
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
€65,00 – €370,00
| Product Name: | ZO-1 Rabbit pAb |
| Category: | Polyclonal Antibodies |
| Description: | This gene encodes a protein located on a cytoplasmic membrane surface of intercellular tight junctions. The encoded protein may be involved in signal transduction at cell-cell junctions. Alternative splicing of this gene results in multiple transcript variants. |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 1600-1700 of human ZO-1 (NP_003248.3). |
| Gene ID: | 7082 |
| Swiss prot: | Q07157 |
| Molecular weight: | 186kDa/195kDa |
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