Western blot analysis of extracts of various cell lines, using PPP1R12B antibody (A17510) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 90s.
PPP1R12B Rabbit pAb
Catalog No:AB-A17510
Manufacturer:ABclonal
Synonyms:MYPT2;PP1bp55;PPP1R12B
Applications:WB
Reactivity:Human
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | PPP1R12B Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | Myosin phosphatase is a protein complex comprised of three subunits: a catalytic subunit (PP1c-delta, protein phosphatase 1, catalytic subunit delta), a large regulatory subunit (MYPT, myosin phosphatase target) and small regulatory subunit (sm-M20). Two isoforms of MYPT have been isolated–MYPT1 and MYPT2, the first of which is widely expressed, and the second of which may be specific to heart, skeletal muscle, and brain. Each of the MYPT isoforms functions to bind PP1c-delta and increase phosphatase activity. This locus encodes both MYTP2 and M20. Alternatively spliced transcript variants encoding different isoforms have been identified. Related pseudogenes have been defined on the Y chromosome. [provided by RefSeq, Oct 2011] |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 660-930 of human PPP1R12B (NP_002472.2). |
Gene ID: | 4660 |
Swiss prot: | O60237 |
Molecular weight: |
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