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Western blot analysis of extracts of various cell lines, using HDAC5 antibody (A0632) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 30s.
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Immunohistochemistry of paraffin-embedded rat liver using HDAC5 antibody (A0632) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human tonsil using HDAC5 antibody (A0632) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse spinal cord using HDAC5 antibody (A0632) at dilution of 1:100 (40x lens).
HDAC5 Rabbit pAb
Catalog No：AB-A0632
Manufacturer：ABclonal
Synonyms：HDAC5;HD5;NY-CO-9
Applications：WB,IHC,IF
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
€65,00 – €370,00
| Product Name: | HDAC5 Rabbit pAb |
| Category: | Polyclonal Antibodies |
| Description: | Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to the class II histone deacetylase/acuc/apha family. It possesses histone deacetylase activity and represses transcription when tethered to a promoter. It coimmunoprecipitates only with HDAC3 family member and might form multicomplex proteins. It also interacts with myocyte enhancer factor-2 (MEF2) proteins, resulting in repression of MEF2-dependent genes. This gene is thought to be associated with colon cancer. Two transcript variants encoding different isoforms have been found for this gene. |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 550-650 of human HDAC5 (NP_005465.2). |
| Gene ID: | 10014 |
| Swiss prot: | Q9UQL6 |
| Molecular weight: | 112kDa/121kDa/122kDa |
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