Western blot analysis of extracts of various cell lines, using RUNX1T1 antibody (A1737) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 1s.
RUNX1T1 Rabbit pAb
Catalog No:AB-A1737
Manufacturer:ABclonal
Synonyms:RUNX1T1;AML1-MTG8;AML1T1;CBFA2T1;CDR;ETO;MTG8;ZMYND2
Applications:WB
Reactivity:Human
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | RUNX1T1 Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | This gene encodes a member of the myeloid translocation gene family which interact with DNA-bound transcription factors and recruit a range of corepressors to facilitate transcriptional repression. The t(8;21)(q22;q22) translocation is one of the most frequent karyotypic abnormalities in acute myeloid leukemia. The translocation produces a chimeric gene made up of the 5′-region of the runt-related transcription factor 1 gene fused to the 3′-region of this gene. The chimeric protein is thought to associate with the nuclear corepressor/histone deacetylase complex to block hematopoietic differentiation. Alternative splicing results in multiple transcript variants. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 100-390 of human RUNX1T1 (NP_783554.1). |
Gene ID: | 862 |
Swiss prot: | Q06455 |
Molecular weight: | 44kDa/48kDa/63kDa/64kDa/67kDa/68kDa |
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