Western blot analysis of extracts of RAW264.7 cells, using GSDMD antibody (A17308) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Enhanced Kit (RM00021).|Exposure time: 180s.
Western blot analysis of extracts of various cell lines, using GSDMD antibody (A17308) at 1:1000 dilution.THP-1 cells were treated by PMA/TPA (80 nM) at 37℃ for overnight and LPS (1 μg/ml) at 37℃ for 6 hours.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 90s.
GSDMD (Full Length+C terminal) Rabbit pAb
Catalog No:AB-A17308
Manufacturer:ABclonal
Synonyms:GSDMD;DF5L;DFNA5L;FKSG10;GSDMDC1
Applications:WB
Reactivity:Human,Mouse
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | GSDMD (Full Length+C terminal) Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | Gasdermin D is a member of the gasdermin family. Members of this family appear to play a role in regulation of epithelial proliferation. Gasdermin D has been suggested to act as a tumor suppressor. Alternatively spliced transcript variants have been described. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 276-484 of human GSDMD (NP_079012.3). |
Gene ID: | 79792 |
Swiss prot: | P57764 |
Molecular weight: | 53kDa |
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