Western blot analysis of extracts of various cell lines, using EIF3A antibody (A0573) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 30s.
EIF3A Rabbit pAb
Catalog No:AB-A0573
Manufacturer:ABclonal
Synonyms:EIF3A;EIF3;EIF3S10;P167;TIF32;eIF3-p170;eIF3-theta;p180;p185
Applications:WB,IHC,IF
Reactivity:Human,Mouse
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | EIF3A Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | RNA-binding component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis (PubMed:17581632, PubMed:25849773). The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation (PubMed:17581632, PubMed:11169732). The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression (PubMed:25849773, PubMed:27462815). |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human EIF3A (NP_003741.1). |
Gene ID: | 8661 |
Swiss prot: | Q14152 |
Molecular weight: | 162kDa/166kDa |
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