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Western blot analysis of extracts of HT-29 cells, using SEMA4A Rabbit pAb (A17205) at 1:3000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 90s.
SEMA4A Rabbit pAb
Catalog No：AB-A17205
Manufacturer：ABclonal
Synonyms：SEMA4A;CORD10;RP35;SEMAB;SEMB
Applications：WB,IF
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
€65,00 – €370,00
| Product Name: | SEMA4A Rabbit pAb |
| Category: | Polyclonal Antibodies |
| Description: | This gene encodes a member of the semaphorin family of soluble and transmembrane proteins. Semaphorins are involved in numerous functions, including axon guidance, morphogenesis, carcinogenesis, and immunomodulation. The encoded protein is a single-pass type I membrane protein containing an immunoglobulin-like C2-type domain, a PSI domain and a sema domain. It inhibits axonal extension by providing local signals to specify territories inaccessible for growing axons. It is an activator of T-cell-mediated immunity and suppresses vascular endothelial growth factor (VEGF)-mediated endothelial cell migration and proliferation in vitro and angiogenesis in vivo. Mutations in this gene are associated with retinal degenerative diseases including retinitis pigmentosa type 35 (RP35) and cone-rod dystrophy type 10 (CORD10). Multiple alternatively spliced transcript variants encoding different isoforms have been identified. |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 320-580 of human SEMA4A (NP_071762.2). |
| Gene ID: | 64218 |
| Swiss prot: | Q9H3S1 |
| Molecular weight: | 69kDa/83kDa |
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