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Western blot analysis of extracts of MCF7 cells, using SOX2 antibody (A0561) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 60s.
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Western blot analysis of extracts of various cell lines, using SOX2 antibody (A0561) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 180s.
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Western blot analysis of extracts from normal (control) and SOX2 knockout (KO) 293T cells, using SOX2 antibody (A0561) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 60s.
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Immunohistochemistry of paraffin-embedded human esophageal using [KO Validated] SOX2 Rabbit pAb (A0561) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse embryos using [KO Validated] SOX2 Rabbit pAb (A0561) at dilution of 1:100 (40x lens).
[KO Validated] SOX2 Rabbit pAb
Catalog No：AB-A0561
Manufacturer：ABclonal
Synonyms：ANOP3;MCOPS3;SOX2;SRY-box 2
Applications：WB,IHC,IF,IP
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
€65,00 – €370,00
| Product Name: | [KO Validated] SOX2 Rabbit pAb |
| Category: | Polyclonal Antibodies |
| Description: | This intronless gene encodes a member of the SRY-related HMG-box (SOX) family of transcription factors involved in the regulation of embryonic development and in the determination of cell fate. The product of this gene is required for stem-cell maintenance in the central nervous system, and also regulates gene expression in the stomach. Mutations in this gene have been associated with optic nerve hypoplasia and with syndromic microphthalmia, a severe form of structural eye malformation. This gene lies within an intron of another gene called SOX2 overlapping transcript (SOX2OT). |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-140 of human SOX2 (NP_003097.1). |
| Gene ID: | 6657 |
| Swiss prot: | P48431 |
| Molecular weight: | 34kDa |
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