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Western blot analysis of extracts of various cell lines, using PUMA antibody (A17138) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 30s.
PUMA Rabbit pAb
Catalog No：AB-A17138
Manufacturer：ABclonal
Synonyms：BBC3;JFY-1;JFY1;PUMA
Applications：WB,IHC,IF
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
€65,00 – €370,00
| Product Name: | PUMA Rabbit pAb |
| Category: | Polyclonal Antibodies |
| Description: | This gene encodes a member of the BCL-2 family of proteins. This family member belongs to the BH3-only pro-apoptotic subclass. The protein cooperates with direct activator proteins to induce mitochondrial outer membrane permeabilization and apoptosis. It can bind to anti-apoptotic Bcl-2 family members to induce mitochondrial dysfunction and caspase activation. Because of its pro-apoptotic role, this gene is a potential drug target for cancer therapy and for tissue injury. Alternative splicing results in multiple transcript variants. |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 50 to the C-terminus of human PUMA (NP_001120713.1). |
| Gene ID: | 27113 |
| Swiss prot: | Q96PG8/Q9BXH1 |
| Molecular weight: | 10kDa/14kDa/20kDa/26kDa |
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