Western blot analysis of extracts of various cell lines, using CENPB antibody (A16817) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 5min.
CENPB Rabbit pAb
Catalog No:AB-A16817
Manufacturer:ABclonal
Synonyms:CENPB
Applications:WB
Reactivity:Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | CENPB Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | This gene product is a highly conserved protein that facilitates centromere formation. It is a DNA-binding protein that is derived from transposases of the pogo DNA transposon family. It contains a helix-loop-helix DNA binding motif at the N-terminus, and a dimerization domain at the C-terminus. The DNA binding domain recognizes and binds a 17-bp sequence (CENP-B box) in the centromeric alpha satellite DNA. This protein is proposed to play an important role in the assembly of specific centromere structures in interphase nuclei and on mitotic chromosomes. It is also considered a major centromere autoantigen recognized by sera from patients with anti-centromere antibodies. |
Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 500 to the C-terminus of human CENPB (NP_001801.1). |
Gene ID: | 1059 |
Swiss prot: | P07199 |
Molecular weight: |
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